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1.
Int. braz. j. urol ; 47(2): 322-332, Mar.-Apr. 2021. tab, graf
Artículo en Inglés | LILACS | ID: biblio-1154465

RESUMEN

ABSTRACT Purpose: We assessed the efficacy and safety of a single injection of three bulking agents over the short- and long-term follow-ups in rabbits. Dermal and preputial matrices were compared with Deflux (DxHA) injection. Material and methods: Twenty-four rabbits were divided into three groups. Group I (n=8) underwent the injection of a lyophilized dermal matrix (LDM) beneath the seromuscular layer of the bladder wall. Rabbits in group II (n=8) were injected with lyophilized preputial matrix (LPM). Rabbits of group III (n=8) were injected with DxHA as the control group. They were followed up for 1 and 6 months after the injection. Subcutaneous injection of all bulking agents was also performed in nude mice. Biopsies were stained with LCA (leukocyte common antibody), CD68, CD31, and CD34. Scanning electron microscopy (SEM) and MTT assay were also performed. Results: Immunohistochemistry staining with CD68 and LCA revealed higher inflammation grade in LDM as compared with LPM and DxHA. Fibrosis grade was also higher in LDM both in short- and long-term follow-ups. However, no significant difference was detected in CD31 and CD34 staining between control and experimental groups. SEM analysis showed that the particle size of LPM was more similar to DxHA. MTT assay revealed that cell proliferation was similar in DxHA, LDM, and LPM. In-vivo assay in nude mice model showed more promising results in LPM as compared with LDM. Conclusion: The long-term results demonstrated that LPM was more similar to Deflux with the least local tissue reaction, inflammation, and fibrosis grade.


Asunto(s)
Animales , Dextranos , Ácido Hialurónico , Conejos , Vejiga Urinaria , Inyecciones , Ratones , Ratones Desnudos
2.
Int. braz. j. urol ; 44(3): 608-616, May-June 2018. tab, graf
Artículo en Inglés | LILACS | ID: biblio-954055

RESUMEN

ABSTRACT Purpose: To identify the fetal stem cell (FSC) response to maternal renal injury with emphasis on renal integrity improvement and Y chromosome detection in damaged maternal kidney. Materials and Methods: Eight non-green fluorescent protein (GFP) transgenic Sprague-Dawley rats were mated with GFP-positive transgenic male rats. Renal damage was induced on the right kidney at gestational day 11. The same procedure was performed in eight non-pregnant rats as control group. Three months after delivery, right ne- phrectomy was performed in order to evaluate the injured kidney. The fresh perfused kidneys were stained with anti-GFP antibody. Polymerase chain reaction (PCR) assay was also performed for the Y chromosome detection. Cell culture was performed to detect the GFP-positive cells. Technetium-99m-DMSA renal scan and single-photon emission computed tomography (SPECT) were performed after renal damage induction and 3 months later to evaluate the improvement of renal integrity. Results: The presence of FSCs was confirmed by immune histochemical staining as well as immunofluorescent imaging of the damaged part. Gradient PCR of female rat purified DNA demonstrated the presence of Y-chromosome in the damaged maternal kidney. Moreover, the culture of kidney cells showed GPF- positive cells by immuno- fluorescence microscopy. The acute renal scar was repaired and the integrity of dam- aged kidney reached to near normal levels in experimental group as shown in DMSA scan. However, no significant improvement was observed in control group. Conclusion: FSC seems to be the main mechanism in repairing of the maternal renal injury during pregnancy as indicated by Y chromosome and GFP-positive cells in the sub-cultured medium.


Asunto(s)
Animales , Masculino , Femenino , Embarazo , Cicatrización de Heridas/fisiología , Quimerismo , Células Madre Fetales/fisiología , Enfermedades Renales/fisiopatología , Intercambio Materno-Fetal/fisiología , Factores de Tiempo , Cromosoma Y , Inmunohistoquímica , Tomografía Computarizada de Emisión de Fotón Único , Células Cultivadas , Reacción en Cadena de la Polimerasa , Técnica del Anticuerpo Fluorescente , Ratas Sprague-Dawley , Radiofármacos , Ácido Dimercaptosuccínico de Tecnecio Tc 99m , Modelos Animales de Enfermedad , Enfermedades Renales/patología , Enfermedades Renales/diagnóstico por imagen
3.
Int. braz. j. urol ; 44(3): 591-599, May-June 2018. tab, graf
Artículo en Inglés | LILACS | ID: biblio-954040

RESUMEN

ABSTRACT Objective To assess the role of high-barrier plastic wrap in reducing the number and size of polyps, as well as decreasing the inflammation and allergic reactions in exstro- phy cases, and to compare the results with the application of low-barrier wrap. Materials and Methods Eight patients with bladder exstrophy-epispadias complex (BEEC) that had used a low density polyethylene (LDPE) wrap for coverage of the exposed polypoid bladder in preoperative care management were referred. The main complaint of their parents was increase in size and number of polyps. After a period of 2 months using the same wrap and observing the increasing pattern in size of polyps, these patients were recommended to use a high-barrier wrap which is made of polyvinylidene chloride (PVdC), until closure. Patients were monitored for the number and size of polyps before and after the change of barriers. The incidence of para-exstrophy skin infection/inflammation and skin allergy were assessed. Biopsies were taken from the polyps to identify histopathological characteristics of the exposed polyps. Results The high barrier wrap was applied for a mean ± SD duration of 12±2.1 months. Polyps' size and number decreased after 12 months. No allergic reaction was detected in patients after the usage of PVdC; three patients suffered from low-grade skin allergy when LDPE was applied. Also, pre-malignant changes were observed in none of the patients in histopathological examination after the application of PVdC. Conclusion Polyps' size and number and skin allergy may significantly decrease with the use of a high-barrier wrap. Certain PVdC wraps with more integrity and less evaporative permeability may be more "exstrophy-friendly".


Asunto(s)
Humanos , Masculino , Femenino , Lactante , Preescolar , Niño , Pólipos/terapia , Cuidados Preoperatorios/métodos , Extrofia de la Vejiga/cirugía , Polietileno/uso terapéutico , Pólipos/patología , Valores de Referencia , Enfermedades de la Piel/prevención & control , Factores de Tiempo , Biopsia , Cuidados Preoperatorios/instrumentación , Reproducibilidad de los Resultados , Extrofia de la Vejiga/patología , Epispadias/cirugía , Epispadias/patología , Resultado del Tratamiento , Hipersensibilidad/prevención & control
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